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        1. Misc. Services

          Current Location>>Home>> >>Products >>Antibodies >>Misc. Services

          Protein antigen

          The service contentcycle
          Peptide coupling(KLH/BSA/OVA,≤5mg)2-3 days

          Protein antigen

          The service contentcycle
          The gene synthesis3-4 weeks

          PCR gene amplification

          (Provide the template,≤1000bp)
          2-3 weeks
          Carrier to build2-3 weeks
          Small amounts of expression1 week
          A large number of expression and purification2-3 weeks

          Polyclonal antibody production

          The service contentcycle
          The purity and concentration of the protein antigen detection(SDS-PAGE)2-3 days
          Immune serum collected before1 day
          Animal immunity and serum collection(Two New Zealand rabbit)10 week
          Animal immunity and serum collection(Two New Zealand rabbit,A rapid process)6-8 weeks
          Immune serum titer detection(ELISA)2-3 days
          Immune animal screening before(ELISA)2-3 days
          Immune animal screening before(Western Blot)1 week
          Immune animal screening before(Dot Blot)1 week
          Increase the immune rabbit(1 only)6-10 weeks
          Immune rabbit keep(1 only)1 week
          Increased serum collection(10ml,1 only)1 week
          Extend the immunity and serum collected(10ml,1 only)1 week
          Extend the immunity and serum collected(A goat)20 week
          Extend the immunity and serum collected(Two rats)10 week
          Extend the immunity and serum collected(Four mice)10 week
          Extend the immunity and serum collected(Two guinea pigs)11 week

          Monoclonal antibody production

          The service contentcycle
          The purity and concentration of the protein antigen detection(SDS-PAGE)2-3 days
          Immune serum collected before1 day
          Animal immunity and serum collection(Four mice)6-10 weeks
          Immune serum titer detection(ELISA)2-3 days
          Cell fusion and hybridoma preparation10-16 weeks
          Subtype identification1 week
          Ascites production(Three mice)3-4 weeks
          Transfer cells flask culture(1L)5-7 weeks

          Immune animal screening before

          (ELISA)
          2-3 days

          Immune animal screening before

          (Western Blot)
          1 week

          Immune animal screening before

          (Dot Blot)
          1 week
          Cloning maintain training(1 only)1 week
          Cloning of cryopreserved(1 only)1 week

          Antibody purification

          The service contentcycle
          Saturated ammonium sulfate purification antiserum(≤60ml)3-5 days
          Protein A/G affinity purification antiserum(≤60ml)3-5 days
          Antigen affinity column preparation2-3 days
          Antigen affinity purification antiserum(≤60ml)3-5 days

          Antibody detection

          The service contentcycle
          ELISA detection(1-10 samples)2-3 days
          Cell protein extraction(1 as a sample)2-3 days
          Tissue protein extraction(1 as a sample)2-3 days

          Determination of protein concentration

          (1 as a sample)
          2-3 days
          Antibodies to Western Blot detection(1 as a sample)1 week
          Antibodies are made in Western Blot detection(

          2-8 samples, provide testing sample concentration

          1 week
          Antibodies are made in Western Blot detection(2-8 samples, the customer does not provide samples)2-3 weeks
          On the basis of internal antibody detection, Western Blot detection target antibody(2-8 samples)1-2 weeks
          Dot Blot test(1-10 samples)2-3 days

          IHC testingProvide slices, 1 picture

          1 week

          Antibodies to tag

          The service contentcycle
          HRP labeling(≤5mg)1-2 weeks

          Biotin labeling(≤5mg)

          1-2 weeks


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